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STEMCELL Technologies Inc immunomagnetic negative selection human nk cell enrichment kit

The influence of <t>NK</t> <t>cell</t> education via KIR2DL1 (2DL1) and KIR2DL2/3 (2DL2/3) on the frequency of granzyme B positive (%GzB + ) target cells generated in the ADCC‐GTL assay . (A) The %GzB + cells generated by PBMC from 2DL1 + individuals co‐expressing HLA‐C2 (i.e., educated) or not (i.e., uneducated HLA‐C1 homozygotes). (B) The %GzB + cells generated by PBMC from 2DL2/3 + individuals co‐expressing HLA‐C1 (i.e., educated) or not (i.e., uneducated, HLA‐C2 homozygotes,). (C) The %GzB + cells generated by PBMC from individuals expressing the 3 possible HLA‐C genotypes. C2* = both homozygotes and heterozygotes for C2; C1* = both homozygotes and heterozygotes for C1. The results presented in this figure are a re‐analysis of data previously published in reference

Immunomagnetic Negative Selection Human Nk Cell Enrichment Kit, supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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1) Product Images from "Differential contribution of education through KIR2DL1, KIR2DL3, and KIR3DL1 to antibody‐dependent (AD) NK cell activation and ADCC"

Article Title: Differential contribution of education through KIR2DL1, KIR2DL3, and KIR3DL1 to antibody‐dependent (AD) NK cell activation and ADCC

Journal: Journal of Leukocyte Biology

doi: 10.1002/JLB.4A0617-242RRR

The influence of NK cell education via KIR2DL1 (2DL1) and KIR2DL2/3 (2DL2/3) on the frequency of granzyme B positive (%GzB + ) target cells generated in the ADCC‐GTL assay . (A) The %GzB + cells generated by PBMC from 2DL1 + individuals co‐expressing HLA‐C2 (i.e., educated) or not (i.e., uneducated HLA‐C1 homozygotes). (B) The %GzB + cells generated by PBMC from 2DL2/3 + individuals co‐expressing HLA‐C1 (i.e., educated) or not (i.e., uneducated, HLA‐C2 homozygotes,). (C) The %GzB + cells generated by PBMC from individuals expressing the 3 possible HLA‐C genotypes. C2* = both homozygotes and heterozygotes for C2; C1* = both homozygotes and heterozygotes for C1. The results presented in this figure are a re‐analysis of data previously published in reference

Figure Legend Snippet: The influence of NK cell education via KIR2DL1 (2DL1) and KIR2DL2/3 (2DL2/3) on the frequency of granzyme B positive (%GzB + ) target cells generated in the ADCC‐GTL assay . (A) The %GzB + cells generated by PBMC from 2DL1 + individuals co‐expressing HLA‐C2 (i.e., educated) or not (i.e., uneducated HLA‐C1 homozygotes). (B) The %GzB + cells generated by PBMC from 2DL2/3 + individuals co‐expressing HLA‐C1 (i.e., educated) or not (i.e., uneducated, HLA‐C2 homozygotes,). (C) The %GzB + cells generated by PBMC from individuals expressing the 3 possible HLA‐C genotypes. C2* = both homozygotes and heterozygotes for C2; C1* = both homozygotes and heterozygotes for C1. The results presented in this figure are a re‐analysis of data previously published in reference

Techniques Used: Generated, Expressing

The influence of NK cell education status of NK populations single positive for inhibitory NK receptors (SPiNKRs) on the frequency of granzyme B positive (%GzB + ) target cells generated in ADCC‐GTL assays . Panels A‐C show the %GzB + CEM cells on the y ‐axis generated in an ADCC‐GTL assay using SPiNKR to self‐HLA (i.e., educated) and nonself‐HLA (uneducated) as effector cells. SPiNKR effectors cells were KIR2DL1 + (2DL1) (A), KIR2DL3 + (2DL3) (B) and KIR3DL1 + (3DL1) (C). Bar heights and error bars represent the median and interquartile range %GzB + CEMs for each data set. In panels D‐I, the y ‐axis shows the %GzB + CEM cells generated in an ADCC‐GTL assay using effector cells that were self‐SPiNKR + (D‐F) or nonself‐SPiNKR (G‐I) compared to SPNKG2A + cells and total NK cells from the same individual tested at the same effector to target cell ratios. SPiNKR effector cells were 2DL1 + (D, G), 2DL3 + (E, H) and 3DL1 + (F, I). Paired analyses (Friedman tests) compared within‐individual values for the %GzB + CEM cells

Figure Legend Snippet: The influence of NK cell education status of NK populations single positive for inhibitory NK receptors (SPiNKRs) on the frequency of granzyme B positive (%GzB + ) target cells generated in ADCC‐GTL assays . Panels A‐C show the %GzB + CEM cells on the y ‐axis generated in an ADCC‐GTL assay using SPiNKR to self‐HLA (i.e., educated) and nonself‐HLA (uneducated) as effector cells. SPiNKR effectors cells were KIR2DL1 + (2DL1) (A), KIR2DL3 + (2DL3) (B) and KIR3DL1 + (3DL1) (C). Bar heights and error bars represent the median and interquartile range %GzB + CEMs for each data set. In panels D‐I, the y ‐axis shows the %GzB + CEM cells generated in an ADCC‐GTL assay using effector cells that were self‐SPiNKR + (D‐F) or nonself‐SPiNKR (G‐I) compared to SPNKG2A + cells and total NK cells from the same individual tested at the same effector to target cell ratios. SPiNKR effector cells were 2DL1 + (D, G), 2DL3 + (E, H) and 3DL1 + (F, I). Paired analyses (Friedman tests) compared within‐individual values for the %GzB + CEM cells

Techniques Used: Generated

The mean fluorescence intensity (MFI) of intracellular granzyme B (GzB) and perforin in NK populations single positive for inhibitory Killer Immunoglobulin‐like receptors (SPiKIRs) to self‐SPiKIR (educated) versus nonself‐iKIR (uneducated) . The y ‐axis shows the MFI of intracellular GzB (A‐D) and perforin (D‐F) in S‐SPiKIR + and NS‐SPiKIR + NK cell subset effector cells positive for KIR2DL1 (2DL1) (A, E), KIR2DL3 (2DL3) (B, F) and KIR3DL1 (3DL1) (C, G) and all 3 iKIR combined (D, H). Bar heights and error bars represent the median and interquartile range of GzB and perforin MFI. Mann‐Whitney tests were used to compare groups. No significant between group differences were found

Figure Legend Snippet: The mean fluorescence intensity (MFI) of intracellular granzyme B (GzB) and perforin in NK populations single positive for inhibitory Killer Immunoglobulin‐like receptors (SPiKIRs) to self‐SPiKIR (educated) versus nonself‐iKIR (uneducated) . The y ‐axis shows the MFI of intracellular GzB (A‐D) and perforin (D‐F) in S‐SPiKIR + and NS‐SPiKIR + NK cell subset effector cells positive for KIR2DL1 (2DL1) (A, E), KIR2DL3 (2DL3) (B, F) and KIR3DL1 (3DL1) (C, G) and all 3 iKIR combined (D, H). Bar heights and error bars represent the median and interquartile range of GzB and perforin MFI. Mann‐Whitney tests were used to compare groups. No significant between group differences were found

Techniques Used: Fluorescence, MANN-WHITNEY

Anti‐HIV antibody‐dependent NK cell activation (ADNKA) induced responses in CD56 Bright NKG2A + and CD56 Dim NK cell populations . The frequency of ADNKA in NK cell populations characterized by total responsiveness (A), tri‐functional (B), CD107a + IFN‐γ + (C) and CD107a + (D) response profiles is shown on the y ‐axis for CD56 Dim 2DL1, 2DL2/S2, 2DL3, 3DL1, NKG2A and CD56 Bright NKG2A populations. The percent of all functional cells (%Fxn) was defined as the sum of the frequencies of all possible combinations of CD107a expression and IFN‐γ and CCL4 secretion. Given the low total responsiveness of the CD56 Bright NKG2A population, it was excluded from further analysis. Each data point represents a single individual. Bar heights and error bars represent the median and interquartile range for each group. * P < 0.05, ** P < 0.01, *** P < 0.001

Figure Legend Snippet: Anti‐HIV antibody‐dependent NK cell activation (ADNKA) induced responses in CD56 Bright NKG2A + and CD56 Dim NK cell populations . The frequency of ADNKA in NK cell populations characterized by total responsiveness (A), tri‐functional (B), CD107a + IFN‐γ + (C) and CD107a + (D) response profiles is shown on the y ‐axis for CD56 Dim 2DL1, 2DL2/S2, 2DL3, 3DL1, NKG2A and CD56 Bright NKG2A populations. The percent of all functional cells (%Fxn) was defined as the sum of the frequencies of all possible combinations of CD107a expression and IFN‐γ and CCL4 secretion. Given the low total responsiveness of the CD56 Bright NKG2A population, it was excluded from further analysis. Each data point represents a single individual. Bar heights and error bars represent the median and interquartile range for each group. * P < 0.05, ** P < 0.01, *** P < 0.001

Techniques Used: Activation Assay, Functional Assay, Expressing

Antibody‐dependent NK cell activation (ADNKA) of stimulated CD56 Dim single positive (SP) KIR3DS1 + (SP3DL1 + ), KIR2DL1 + (SP2DL1 + ) and KIR2DL3 + (SP2DL3 + ) NK cells from carriers of HLA allotypes able to educate, versus not, NK cells through these Inhibitory Killer Immunoglobulin‐like Receptors (iKIRs) . The y ‐axis shows the frequency of stimulated CD56 Dim SP3DL1 + NK cells from carriers of a Bw4 allele versus Bw6 homozygotes (A‐D), the frequency of stimulated CD56 Dim SP2DL1 + NK cells from carriers of a C2 allele versus C1 homozygotes (E‐H) and the frequency of stimulated CD56 Dim SP2DL3 + NK cells from carriers of a C1 allele versus C2 homozygotes (I‐L) characterized by total responsiveness (A, E, I), total CD107a (B, F, J), total IFN‐γ (C, G, K), and total CCL4 (D, H, L) response profiles. Each data point represents a single individual. The upper and lower limits of the bars and error bars in these bar and whisker plots represents the interquartile range and maximum/minimum values whereas the line in the bars represents the median value for the group. The significance of comparisons of the frequency of ADNKA in NK cell populations educated, versus not, through these iKIRs is show by “*” = P ≤ 0.05 or “**” = P = 0.01). %Fxn = percent of all functional cells; SP = single positive cells expressed the indicated iKIR to the exclusion of all other inhibitory NK cell receptors measured

Figure Legend Snippet: Antibody‐dependent NK cell activation (ADNKA) of stimulated CD56 Dim single positive (SP) KIR3DS1 + (SP3DL1 + ), KIR2DL1 + (SP2DL1 + ) and KIR2DL3 + (SP2DL3 + ) NK cells from carriers of HLA allotypes able to educate, versus not, NK cells through these Inhibitory Killer Immunoglobulin‐like Receptors (iKIRs) . The y ‐axis shows the frequency of stimulated CD56 Dim SP3DL1 + NK cells from carriers of a Bw4 allele versus Bw6 homozygotes (A‐D), the frequency of stimulated CD56 Dim SP2DL1 + NK cells from carriers of a C2 allele versus C1 homozygotes (E‐H) and the frequency of stimulated CD56 Dim SP2DL3 + NK cells from carriers of a C1 allele versus C2 homozygotes (I‐L) characterized by total responsiveness (A, E, I), total CD107a (B, F, J), total IFN‐γ (C, G, K), and total CCL4 (D, H, L) response profiles. Each data point represents a single individual. The upper and lower limits of the bars and error bars in these bar and whisker plots represents the interquartile range and maximum/minimum values whereas the line in the bars represents the median value for the group. The significance of comparisons of the frequency of ADNKA in NK cell populations educated, versus not, through these iKIRs is show by “*” = P ≤ 0.05 or “**” = P = 0.01). %Fxn = percent of all functional cells; SP = single positive cells expressed the indicated iKIR to the exclusion of all other inhibitory NK cell receptors measured

Techniques Used: Activation Assay, Whisker Assay, Functional Assay

Isolation:

Article Title: Differential contribution of education through KIR2DL1, KIR2DL3, and KIR3DL1 to antibody‐dependent (AD) NK cell activation and ADCC
Article Snippet: .. NK cells were isolated from 1 × 109 PBMCs using an immunomagnetic negative selection human NK cell enrichment kit (STEMCELL Technologies, Vancouver, British Columbia, Canada). ..

Article Title: Differential contribution of education through KIR2DL1, KIR2DL3, and KIR3DL1 to antibody‐dependent (AD) NK cell activation and ADCC
Article Snippet: .. NK cells were isolated from 1 × 10 9 PBMCs using an immunomagnetic negative selection human NK cell enrichment kit (STEMCELL Technologies, Vancouver, British Columbia, Canada). ..

Selection:

Article Title: Differential contribution of education through KIR2DL1, KIR2DL3, and KIR3DL1 to antibody‐dependent (AD) NK cell activation and ADCC
Article Snippet: .. NK cells were isolated from 1 × 10 9 PBMCs using an immunomagnetic negative selection human NK cell enrichment kit (STEMCELL Technologies, Vancouver, British Columbia, Canada). ..

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Journal: Journal of Leukocyte Biology

Article Title: Differential contribution of education through KIR2DL1, KIR2DL3, and KIR3DL1 to antibody‐dependent (AD) NK cell activation and ADCC

doi: 10.1002/JLB.4A0617-242RRR

Figure Lengend Snippet: The influence of NK cell education via KIR2DL1 (2DL1) and KIR2DL2/3 (2DL2/3) on the frequency of granzyme B positive (%GzB + ) target cells generated in the ADCC‐GTL assay . (A) The %GzB + cells generated by PBMC from 2DL1 + individuals co‐expressing HLA‐C2 (i.e., educated) or not (i.e., uneducated HLA‐C1 homozygotes). (B) The %GzB + cells generated by PBMC from 2DL2/3 + individuals co‐expressing HLA‐C1 (i.e., educated) or not (i.e., uneducated, HLA‐C2 homozygotes,). (C) The %GzB + cells generated by PBMC from individuals expressing the 3 possible HLA‐C genotypes. C2* = both homozygotes and heterozygotes for C2; C1* = both homozygotes and heterozygotes for C1. The results presented in this figure are a re‐analysis of data previously published in reference

Article Snippet: NK cells were isolated from 1 × 10 9 PBMCs using an immunomagnetic negative selection human NK cell enrichment kit (STEMCELL Technologies, Vancouver, British Columbia, Canada).

Techniques: Generated, Expressing

Journal: Journal of Leukocyte Biology

Article Title: Differential contribution of education through KIR2DL1, KIR2DL3, and KIR3DL1 to antibody‐dependent (AD) NK cell activation and ADCC

doi: 10.1002/JLB.4A0617-242RRR

Figure Lengend Snippet: The influence of NK cell education status of NK populations single positive for inhibitory NK receptors (SPiNKRs) on the frequency of granzyme B positive (%GzB + ) target cells generated in ADCC‐GTL assays . Panels A‐C show the %GzB + CEM cells on the y ‐axis generated in an ADCC‐GTL assay using SPiNKR to self‐HLA (i.e., educated) and nonself‐HLA (uneducated) as effector cells. SPiNKR effectors cells were KIR2DL1 + (2DL1) (A), KIR2DL3 + (2DL3) (B) and KIR3DL1 + (3DL1) (C). Bar heights and error bars represent the median and interquartile range %GzB + CEMs for each data set. In panels D‐I, the y ‐axis shows the %GzB + CEM cells generated in an ADCC‐GTL assay using effector cells that were self‐SPiNKR + (D‐F) or nonself‐SPiNKR (G‐I) compared to SPNKG2A + cells and total NK cells from the same individual tested at the same effector to target cell ratios. SPiNKR effector cells were 2DL1 + (D, G), 2DL3 + (E, H) and 3DL1 + (F, I). Paired analyses (Friedman tests) compared within‐individual values for the %GzB + CEM cells

Article Snippet: NK cells were isolated from 1 × 10 9 PBMCs using an immunomagnetic negative selection human NK cell enrichment kit (STEMCELL Technologies, Vancouver, British Columbia, Canada).

Techniques: Generated

Journal: Journal of Leukocyte Biology

Article Title: Differential contribution of education through KIR2DL1, KIR2DL3, and KIR3DL1 to antibody‐dependent (AD) NK cell activation and ADCC

doi: 10.1002/JLB.4A0617-242RRR

Figure Lengend Snippet: The mean fluorescence intensity (MFI) of intracellular granzyme B (GzB) and perforin in NK populations single positive for inhibitory Killer Immunoglobulin‐like receptors (SPiKIRs) to self‐SPiKIR (educated) versus nonself‐iKIR (uneducated) . The y ‐axis shows the MFI of intracellular GzB (A‐D) and perforin (D‐F) in S‐SPiKIR + and NS‐SPiKIR + NK cell subset effector cells positive for KIR2DL1 (2DL1) (A, E), KIR2DL3 (2DL3) (B, F) and KIR3DL1 (3DL1) (C, G) and all 3 iKIR combined (D, H). Bar heights and error bars represent the median and interquartile range of GzB and perforin MFI. Mann‐Whitney tests were used to compare groups. No significant between group differences were found

Article Snippet: NK cells were isolated from 1 × 10 9 PBMCs using an immunomagnetic negative selection human NK cell enrichment kit (STEMCELL Technologies, Vancouver, British Columbia, Canada).

Techniques: Fluorescence, MANN-WHITNEY

Journal: Journal of Leukocyte Biology

Article Title: Differential contribution of education through KIR2DL1, KIR2DL3, and KIR3DL1 to antibody‐dependent (AD) NK cell activation and ADCC

doi: 10.1002/JLB.4A0617-242RRR

Figure Lengend Snippet: Anti‐HIV antibody‐dependent NK cell activation (ADNKA) induced responses in CD56 Bright NKG2A + and CD56 Dim NK cell populations . The frequency of ADNKA in NK cell populations characterized by total responsiveness (A), tri‐functional (B), CD107a + IFN‐γ + (C) and CD107a + (D) response profiles is shown on the y ‐axis for CD56 Dim 2DL1, 2DL2/S2, 2DL3, 3DL1, NKG2A and CD56 Bright NKG2A populations. The percent of all functional cells (%Fxn) was defined as the sum of the frequencies of all possible combinations of CD107a expression and IFN‐γ and CCL4 secretion. Given the low total responsiveness of the CD56 Bright NKG2A population, it was excluded from further analysis. Each data point represents a single individual. Bar heights and error bars represent the median and interquartile range for each group. * P < 0.05, ** P < 0.01, *** P < 0.001

Article Snippet: NK cells were isolated from 1 × 10 9 PBMCs using an immunomagnetic negative selection human NK cell enrichment kit (STEMCELL Technologies, Vancouver, British Columbia, Canada).

Techniques: Activation Assay, Functional Assay, Expressing

Journal: Journal of Leukocyte Biology

Article Title: Differential contribution of education through KIR2DL1, KIR2DL3, and KIR3DL1 to antibody‐dependent (AD) NK cell activation and ADCC

doi: 10.1002/JLB.4A0617-242RRR

Figure Lengend Snippet: Antibody‐dependent NK cell activation (ADNKA) of stimulated CD56 Dim single positive (SP) KIR3DS1 + (SP3DL1 + ), KIR2DL1 + (SP2DL1 + ) and KIR2DL3 + (SP2DL3 + ) NK cells from carriers of HLA allotypes able to educate, versus not, NK cells through these Inhibitory Killer Immunoglobulin‐like Receptors (iKIRs) . The y ‐axis shows the frequency of stimulated CD56 Dim SP3DL1 + NK cells from carriers of a Bw4 allele versus Bw6 homozygotes (A‐D), the frequency of stimulated CD56 Dim SP2DL1 + NK cells from carriers of a C2 allele versus C1 homozygotes (E‐H) and the frequency of stimulated CD56 Dim SP2DL3 + NK cells from carriers of a C1 allele versus C2 homozygotes (I‐L) characterized by total responsiveness (A, E, I), total CD107a (B, F, J), total IFN‐γ (C, G, K), and total CCL4 (D, H, L) response profiles. Each data point represents a single individual. The upper and lower limits of the bars and error bars in these bar and whisker plots represents the interquartile range and maximum/minimum values whereas the line in the bars represents the median value for the group. The significance of comparisons of the frequency of ADNKA in NK cell populations educated, versus not, through these iKIRs is show by “*” = P ≤ 0.05 or “**” = P = 0.01). %Fxn = percent of all functional cells; SP = single positive cells expressed the indicated iKIR to the exclusion of all other inhibitory NK cell receptors measured

Article Snippet: NK cells were isolated from 1 × 10 9 PBMCs using an immunomagnetic negative selection human NK cell enrichment kit (STEMCELL Technologies, Vancouver, British Columbia, Canada).

Techniques: Activation Assay, Whisker Assay, Functional Assay

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immunomagnetic negative selection human nk cell enrichment kit (STEMCELL Technologies Inc)

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